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melanocyte growth media m2  (PromoCell)


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    Structured Review

    PromoCell melanocyte growth media m2
    Melanocyte Growth Media M2, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/melanocyte+growth+media+m2/Melanocyte+Growth+Medium+M2/pmc06680049-205-14-7
    Average 94 stars, based on 1 article reviews
    melanocyte growth media m2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: C-Jun drives melanoma progression in PTEN wild type melanoma cells
    Article Snippet: Human melanoma cell lines Sbcl-2, WM3211, WM1366, WM793, WM1158, and WM9 (a generous gift from Dr. M. Herlyn, Wistar Institute, Philadelphia, USA) derived from RGP (Sbcl-2), VGP (WM3211, WM1366, WM793), and melanoma MET (WM1158, WM9) were maintained in a culture medium consisting of MCDB153 (Sigma-Aldrich, Steinheim, Germany) with 20% Leibovitz’s L-15 (PAA Laboratories, Coelbe, Germany), 2% FCS, 1.68 mM CaCl 2 (Sigma), and 5 μg/ml insulin (Sigma-Aldrich, Steinheim, Germany) at 37 °C and 5% CO 2 . .. NHEM were derived from neonatal foreskin (NHEMs, PromoCell, Heidelberg, Germany) and were cultured in melanocyte growth media M2 at 37 °C and 5% CO 2 . .. Western blot analysis was performed as described previously using one of the following antibodies: anti- c-Jun (1 in 1000 dilution; Cell Signaling, Frankfurt am Main, Germany), anti-GAPDH (1 in 1000 dilution; Cell Signaling), anti ß-Actin (1 in 3000 dilution; Sigma Aldrich), anti- PTEN (1 in 40 dilution; Santa Cruz), anti- AKT (1 in 2000 dilution; Cell Signaling, Frankfurt am Main, Germany), and anti-P- AKT (1 in 2000 dilution; Cell Signaling, Frankfurt am Main, Germany).

    Cell Culture:

    Article Title: C-Jun drives melanoma progression in PTEN wild type melanoma cells
    Article Snippet: Human melanoma cell lines Sbcl-2, WM3211, WM1366, WM793, WM1158, and WM9 (a generous gift from Dr. M. Herlyn, Wistar Institute, Philadelphia, USA) derived from RGP (Sbcl-2), VGP (WM3211, WM1366, WM793), and melanoma MET (WM1158, WM9) were maintained in a culture medium consisting of MCDB153 (Sigma-Aldrich, Steinheim, Germany) with 20% Leibovitz’s L-15 (PAA Laboratories, Coelbe, Germany), 2% FCS, 1.68 mM CaCl 2 (Sigma), and 5 μg/ml insulin (Sigma-Aldrich, Steinheim, Germany) at 37 °C and 5% CO 2 . .. NHEM were derived from neonatal foreskin (NHEMs, PromoCell, Heidelberg, Germany) and were cultured in melanocyte growth media M2 at 37 °C and 5% CO 2 . .. Western blot analysis was performed as described previously using one of the following antibodies: anti- c-Jun (1 in 1000 dilution; Cell Signaling, Frankfurt am Main, Germany), anti-GAPDH (1 in 1000 dilution; Cell Signaling), anti ß-Actin (1 in 3000 dilution; Sigma Aldrich), anti- PTEN (1 in 40 dilution; Santa Cruz), anti- AKT (1 in 2000 dilution; Cell Signaling, Frankfurt am Main, Germany), and anti-P- AKT (1 in 2000 dilution; Cell Signaling, Frankfurt am Main, Germany).



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